| 出品公司: | -- |
|---|---|
| 载体名称: | pGL4.15[luc2P/Hygro] |
| 质粒类型: | 启动子报告载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 5969 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 潮霉素 |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | 编码失稳荧光素酶的无启动子载体，用于测定启动子和增强子序列的活性。 |
| 产品目录号: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGL4.15[luc2P Hygro] 5969 bp ds-DNA circular SYN 29-JUN-2013
DEFINITION Promoterless vector encoding destabilized luciferase for measuring
the activity of promoter and enhancer sequences.
ACCESSION AY864929
VERSION .
KEYWORDS pGL4.15[luc2P Hygro]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5969)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Saturday, December 7, 2019 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5969
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
misc_feature 1..70
/note="MCS"
/note="multiple cloning site"
CDS 100..1749
/codon_start=1
/product="firefly luciferase"
/note="luciferase"
/note="synthetic luc2 version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
CDS 1753..1872
/codon_start=1
/product="PEST degradation sequence from mouse ornithine
decarboxylase"
/note="hPEST"
/note="human codon-optimized"
/translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
polyA_signal 1924..2045
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 2239..2596
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 2627..3664
/codon_start=1
/product="hygromycin B phosphotransferase"
/note="HygR"
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
EVGRV"
polyA_signal 3688..3736
/note="synthetic polyadenylation signal"
rep_origin complement(4063..4651)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4851..5711)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 5816..5864
/note="synthetic polyadenylation signal"
misc_feature 5878..5969
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt
121 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac
181 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc
241 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg
301 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag
361 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac
421 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc
481 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa
541 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc
601 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac
661 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc
721 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc
781 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc
841 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc
901 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg
961 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc
1021 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg
1081 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc
1141 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc
1201 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc
1261 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct
1321 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac
1381 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac
1441 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc
1501 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg
1561 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca
1621 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc
1681 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag
1741 atcgccgtga attctcacgg cttccctccc gaggtggagg agcaggccgc cggcaccctg
1801 cccatgagct gcgcccagga gagcggcatg gatagacacc ctgctgcttg cgccagcgcc
1861 aggatcaacg tctaaggccg cgactctaga gtcggggcgg ccggccgctt cgagcagaca
1921 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
1981 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
2041 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
2101 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtttgcgt
2161 attgggcgct cttccgctga tctgcgcagc accatggcct gaaataacct ctgaaagagg
2221 aacttggtta gctaccttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
2281 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
2341 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
2401 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
2461 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
2521 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
2581 cctaggcttt tgcaaaaagc tcgattcttc tgacactagc gccaccatga agaagcccga
2641 actcaccgct accagcgttg aaaaatttct catcgagaag ttcgacagtg tgagcgacct
2701 gatgcagttg tcggagggcg aagagagccg agccttcagc ttcgatgtcg gcggacgcgg
2761 ctatgtactg cgggtgaata gctgcgctga tggcttctac aaagaccgct acgtgtaccg
2821 ccacttcgcc agcgctgcac tacccatccc cgaagtgttg gacatcggcg agttcagcga
2881 gagcctgaca tactgcatca gtagacgcgc ccaaggcgtt actctccaag acctccccga
2941 aacagagctg cctgctgtgt tacagcctgt cgccgaagct atggatgcta ttgccgccgc
3001 cgacctcagt caaaccagcg gcttcggccc attcgggccc caaggcatcg gccagtacac
3061 aacctggcgg gatttcattt gcgccattgc tgatccccat gtctaccact ggcagaccgt
3121 gatggacgac accgtgtccg ccagcgtagc tcaagccctg gacgaactga tgctgtgggc
3181 cgaagactgt cccgaggtgc gccacctcgt ccatgccgac ttcggcagca acaacgtcct
3241 gaccgacaac ggccgcatca ccgccgtaat cgactggtcc gaagctatgt tcggggacag
3301 tcagtacgag gtggccaaca tcttcttctg gcggccctgg ctggcttgca tggagcagca
3361 gactcgctac ttcgagcgcc ggcatcccga gctggccggc agccctcgtc tgcgagccta
3421 catgctgcgc atcggcctgg atcagctcta ccagagcctc gtggacggca acttcgacga
3481 tgctgcctgg gctcaaggcc gctgcgatgc catcgtccgc agcggggccg gcaccgtcgg
3541 tcgcacacaa atcgctcgcc ggagcgcagc cgtatggacc gacggctgcg tcgaggtgct
3601 ggccgacagc ggcaaccgcc ggcccagtac acgaccgcgc gctaaggagg taggtcgagt
3661 ttaaactcta gaaccggtca tggccgcaat aaaatatctt tattttcatt acatctgtgt
3721 gttggttttt tgtgtgttcg aactagatgc tgtcgaccga tgcccttgag agccttcaac
3781 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
3841 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac
3901 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
3961 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
4021 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
4081 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
4141 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
4201 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
4261 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
4321 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
4381 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
4441 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
4501 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
4561 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
4621 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
4681 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
4741 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
4801 tgagtaaact tggtctgaca gcggccgcaa atgctaaacc actgcagtgg ttaccagtgc
4861 ttgatcagtg aggcaccgat ctcagcgatc tgcctatttc gttcgtccat agtggcctga
4921 ctccccgtcg tgtagatcac tacgattcgt gagggcttac catcaggccc cagcgcagca
4981 atgatgccgc gagagccgcg ttcaccggcc cccgatttgt cagcaatgaa ccagccagca
5041 gggagggccg agcgaagaag tggtcctgct actttgtccg cctccatcca gtctatgagc
5101 tgctgtcgtg atgctagagt aagaagttcg ccagtgagta gtttccgaag agttgtggcc
5161 attgctactg gcatcgtggt atcacgctcg tcgttcggta tggcttcgtt caactctggt
5221 tcccagcggt caagccgggt cacatgatca cccatattat gaagaaatgc agtcagctcc
5281 ttagggcctc cgatcgttgt cagaagtaag ttggccgcgg tgttgtcgct catggtaatg
5341 gcagcactac acaattctct taccgtcatg ccatccgtaa gatgcttttc cgtgaccggc
5401 gagtactcaa ccaagtcgtt ttgtgagtag tgtatacggc gaccaagctg ctcttgcccg
5461 gcgtctatac gggacaacac cgcgccacat agcagtactt tgaaagtgct catcatcggg
5521 aatcgttctt cggggcggaa agactcaagg atcttgccgc tattgagatc cagttcgata
5581 tagcccactc ttgcacccag ttgatcttca gcatctttta ctttcaccag cgtttcgggg
5641 tgtgcaaaaa caggcaagca aaatgccgca aagaagggaa tgagtgcgac acgaaaatgt
5701 tggatgctca tactcgtcct ttttcaatat tattgaagca tttatcaggg ttactagtac
5761 gtctctcaag gataagtaag taatattaag gtacgggagg tattggacag gccgcaataa
5821 aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac
5881 atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag
5941 tgcaagtgca ggtgccagaa catttctct
//
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